Design considerations for murine retinal imaging using scattering angle resolved optical coherence tomography

Authors: Gardner M.R., Katta N., Rahman A.S., Rylander H.G., III, Milner T.E.

Author Affiliations: Gardner, M.R., Department of Biomedical Engineering, The University of Texas at Austin, 107W Dean Keeton St, Austin, TX 78712, United States, Department of Chemical Engineering, University of Bahrain, P.O. Box 32038, Isa Town, Bahrain; Katta, N., Department of Biomedical Engineering, The University of Texas at Austin, 107W Dean Keeton St, Austin, TX 78712, United States; Rahman, A.S., Department of Biomedical Engineering, The University of Texas at Austin, 107W Dean Keeton St, Austin, TX 78712, United States; Rylander, H.G., III, Department of Biomedical Engineering, The University of Texas at Austin, 107W Dean Keeton St, Austin, TX 78712, United States; Milner, T.E., Department of Biomedical Engineering, The University of Texas at Austin, 107W Dean Keeton St, Austin, TX 78712, United States

Publication Date: 2018

Optical coherence tomography (OCT), an optical imaging approach enabling cross-sectional analysis of turbid samples, is routinely used for retinal imaging in human and animal models of diseases affecting the retina. Scattering angle resolved (SAR-)OCT has previously been demonstrated as offering additional contrast in human studies, but no SAR-OCT system has been reported in detail for imaging the retinas of mice. An optical model of a mouse eye was designed and extended for validity at wavelengths of light around 1310 nm; this model was then utilized to develop a SAR-OCT design for murine retinal imaging. A Monte Carlo technique simulates light scattering from the retina, and the simulation results are confirmed with SAR-OCT images. Various images from the SAR-OCT system are presented and utility of the system is described. SAR-OCT is demonstrated as a viable and robust imaging platform to extend utility of retinal OCT imaging by incorporating scattering data into investigative ophthalmologic analysis. © 2018 by the authors.

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Eshan2020-11-28T18:20:26+00:00

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