The sensing characteristics of extended gate field effect transistor (EGFET) as pH sensor based on V2O5 nanorods (NRs) was fabricated for the first time. The as-deposited V2O5 NRs onto glass substrate by using spray pyrolysis method have a diameter of 140–160 nm and a length of 300–500 nm. The V2O5 NRs based EGFET pH sensor exhibited a remarkable pH sensing performance due to the larger effective sensing surface area. The sensing sensitivity and linearity of the pH-EGFET sensor was found to be 54.9 mV/pH and 0.9859, respectively in the range of pH 2–12. V2O5 NRs sensor showed superior pH sensing stability and reliability, thereby can be considered as potential candidate for mass fabrication in disposable biosensors. © 2016, Springer Science+Business Media New York.